US-3D-Implant - „Ultrasound-Driven ex-vivo Design of Tissue-Guiding Constructs for Bone Regeneration”
The goal of the project is to develop an ultrasound-based methodology for the fabrication of mechanically, structurally, and biologically tunable tissue-guiding ex vivo constructs for the regeneration of bone defects. Ultrasound will be used to align, stimulate, and visualize specially designed anisotropic tissue constructs.
The acoustic forces are used
- for the structured organization of mesenchymal stromal cells (MSCs) and bone morphogenetic protein-2 (BMP-2) growth factor encapsulated in liposomes in the in situ polymerization gel.
- to control of the (specific and temporal) release of BMP-2 from the immobilized liposomes.
- for the promotion of cellular survival throughout the construct and
- the reduction of BMP-2 concentration required for efficient osteogenic differentiation of cells
Scanning Acoustic Microscopy (SAM) and High-Frequency Acoustic Biomicroscopy is uesd to
- for the longitudinal imaging of the tissue constructs
- obtain additional information about the evolution of mechanical properties
- Dr. Regina Puts
- Hui Zhang
- B.Sc. Nirina Beilfuß
- DFG Pu701/1-1